Journal: Molecules

Article Title: Replicates Number for Drug Stability Testing during Bioanalytical Method Validation—An Experimental and Retrospective Approach

doi: 10.3390/molecules27020457

Figure Lengend Snippet: Parameters of MS method.

Article Snippet: O-desmethyl-tramadol-d6 (≤98%) and tramadol-d6 hydrochloride (≤99%) were purchased from TLC Pharmaceutical Standards (Newmarket, Ontario, Canada).

Techniques:

Journal: Molecules

Article Title: Replicates Number for Drug Stability Testing during Bioanalytical Method Validation—An Experimental and Retrospective Approach

doi: 10.3390/molecules27020457

Figure Lengend Snippet: The 90% confidence intervals for the stability calculated according to for ( a ) tramadol and ( b ) O-desmethyl-tramadol in human plasma stored at room temperature for 24 h and 72 h. Each sample size is associated with a different color, with light color indicating low concentration and dark color indicating high concentration. Vertical dashed lines indicate stability limits of 85–115%.

Article Snippet: O-desmethyl-tramadol-d6 (≤98%) and tramadol-d6 hydrochloride (≤99%) were purchased from TLC Pharmaceutical Standards (Newmarket, Ontario, Canada).

Techniques: Concentration Assay

Journal: Molecules

Article Title: Replicates Number for Drug Stability Testing during Bioanalytical Method Validation—An Experimental and Retrospective Approach

doi: 10.3390/molecules27020457

Figure Lengend Snippet: Descriptive statistics for the width [%] of a 90% confidence interval. The number of pairs is the equal number of reference and study samples.

Article Snippet: O-desmethyl-tramadol-d6 (≤98%) and tramadol-d6 hydrochloride (≤99%) were purchased from TLC Pharmaceutical Standards (Newmarket, Ontario, Canada).

Techniques:

Journal: Molecules

Article Title: Replicates Number for Drug Stability Testing during Bioanalytical Method Validation—An Experimental and Retrospective Approach

doi: 10.3390/molecules27020457

Figure Lengend Snippet: Width of a 90% confidence interval for stability calculated according to for tramadol (full color) and O-desmethyl-tramadol (striped color) in human plasma stored at room temperature for 24 h and 72 h for each sample size: ( a ) low concentration, ( b ) high concentration.

Article Snippet: O-desmethyl-tramadol-d6 (≤98%) and tramadol-d6 hydrochloride (≤99%) were purchased from TLC Pharmaceutical Standards (Newmarket, Ontario, Canada).

Techniques: Concentration Assay

Journal: Molecules

Article Title: Replicates Number for Drug Stability Testing during Bioanalytical Method Validation—An Experimental and Retrospective Approach

doi: 10.3390/molecules27020457

Figure Lengend Snippet: Post-hoc least significant difference test (Fisher’s LSD test). Vertical bars means a 90% confidence interval: ( a ) tramadol; ( b ) O-desmethyl-tramadol. On each plot, light color indicates low concentration and dark color indicates high concentration.

Article Snippet: O-desmethyl-tramadol-d6 (≤98%) and tramadol-d6 hydrochloride (≤99%) were purchased from TLC Pharmaceutical Standards (Newmarket, Ontario, Canada).

Techniques: Concentration Assay

Journal: Molecules

Article Title: Replicates Number for Drug Stability Testing during Bioanalytical Method Validation—An Experimental and Retrospective Approach

doi: 10.3390/molecules27020457

Figure Lengend Snippet: Concentrations of tramadol in human plasma during stability testing after storage for 24 h and 72 h at room temperature—low QC (nominal concentration of 15.0 ng/mL) and high QC (nominal concentration of 600 ng/mL).

Article Snippet: O-desmethyl-tramadol-d6 (≤98%) and tramadol-d6 hydrochloride (≤99%) were purchased from TLC Pharmaceutical Standards (Newmarket, Ontario, Canada).

Techniques: Concentration Assay

Journal: Molecules

Article Title: Replicates Number for Drug Stability Testing during Bioanalytical Method Validation—An Experimental and Retrospective Approach

doi: 10.3390/molecules27020457

Figure Lengend Snippet: Concentrations of O-desmethyl tramadol in human plasma during stability testing after storage for 24 h and 72 h at room temperature—low QC (nominal concentration of 7.50 ng/mL) and high QC (nominal concentration of 120 ng/mL).

Article Snippet: O-desmethyl-tramadol-d6 (≤98%) and tramadol-d6 hydrochloride (≤99%) were purchased from TLC Pharmaceutical Standards (Newmarket, Ontario, Canada).

Techniques: Concentration Assay